Syrian air defenses responded to a missile strike in the central province of Homs after midnight Tuesday, state news agency SANA reported.
State television showed pictures of a missile that was shot in the air above the air base only days after a US, British and French attack on Syrian targets in retaliation for a suspected chemical attack on the city of Douma on the outskirts of Damascus.
The raid in Homs countryside reportedly coincided with another missile attack against a military airbase near Damascus.
Hezbollah's AlManar TV reported that six missiles targeted Shayrat and three were aimed at Dumair military airport outside Homs on Monday.
Shayrat air base was targeted by US Tomahawk missiles in April a year ago in a strike ordered by President Donald Trump in retaliation for a suspected chemical attack on the rebel-held town of Khan Sheikhun in Idlib.
"There is no US military activity in that area at this time", the US Defense Department said, according to Reuters.
The strikes come less than 72 hours after the US, France and the United Kingdom carried out missile strikes against three targets in Syria, that include two chemical weapons facilities and a research centre for the regime.
Up to 10 missiles were destroyed by the Syrian Armed Forces, a military source told Sputnik.
"We don't comment on such reports", Reuters quoted a spokesman for the Israeli military as saying.