Samuel was capped by England from Under-18 to Under-21 level and was an unused substitute for a senior global against Sweden in 2004.
"Jlloyd was a loving son, father, husband and brother.He was also a respected ex-footballer and businessman who played for a number of clubs.He was extremely popular and well-loved by all who were fortunate to know him".
"At around 7.55am (06:55GMT) police were called following reports of a collision involving a van and a Range Rover", said a statement from Cheshire Police.
Jlloyd Samuel, the former Trinidad and Tobago defender who played in the English Premier League for Aston Villa and Bolton, has died.
The ex-Aston Villa, Bolton and Cardiff defender was involved in the smash moments after dropping his children off at school at around 8am yesterday.
They added: "His next of kin have been informed and are now being supported by specially trained officers". He played two full global matches for Trinidad and Tobago in 2009.
According to police, a man, 54, sustained serious injuries and was taken to hospital after the crash.
He moved to fellow Premier League side Bolton in 2007, where he spent four years, before ending his playing days in Iran.
Samuel leaves behind wife Emma and their three children, aged 10, 8 and 5. "May his soul rest in peace".
Ashley Young, the Manchester United and England player, wrote: "Absolutely gutted to hear the news of the passing of Jlloyd Samuel".
"The TTFA and all his former teammates extends deepest condolences to his family in this most hard time".
Samuel progressed through the ranks at Charlton before joining Aston Villa.