The world's top-ranked ODI team, who play the first of five matches against Australia in London on Wednesday, were beaten by six runs in the 49th over after failing to pull off what would have been the second-highest run chase in ODI history.
Here in this slider, we take a look at five player battles which could determine the result of the match and the series as a whole.
"I'm really confident, feel like I'm bowling quite nicely especially with the white ball", he said.
Scotland's shock one-day worldwide win over England has boosted their chances of securing test-playing status, the country's cricket board has said. England are a brilliant side but scoring 371 against them means the world'.
But Derbyshire have moved quickly to secure the 85-cap worldwide batsman on the back of his seventh ODI hundred as part of a new relationship between the midlands county and Cricket Scotland.
Scotland's victory will send out a powerful message to the ICC, which voted to restrict the 2019 World Cup to 10 teams from the previous 14, which makes it far more hard for second-tier sides to qualify.
England move on to the Oval next where they open a five-match series against Australia on Wednesday, and Morgan felt the shock defeat at the Grange would serve as the ideal warm-up for his players.
Scotland saw captain Kyle Coetzer and Matthew Cross share a century stand after Morgan won the toss.
Paine expects a strong response from England, saying: "I'm sure they'll come back better for what happened in Scotland".
MacLeod, however, ensured a promising start was not wasted and together with George Munsey (55) put on 107 for the fourth wicket.
Victory completed a notable double over England for the Sole family.
The Oval will be just Agar's fifth ODI since he made his debut against England at Old Trafford in 2015 - although English audiences are more likely to remember him for his 98 on Test debut batting at No. 11 in the 2013 Ashes - and he now feels a more rounded cricketer.