Customers started reporting problems at around 5am on Friday and a statement issued later that morning by the bank said the issues had been resolved.
Online a telephone services were offline for at least seven hours, however, Barclays say "everything is now fixed".
A Barclays spokesman said: "We're very sorry about the technical problems our customers have experienced today".
"Thanks for bearing with us". "However, new payees aren't possible at the moment due to the incident".
The bank also acknowledged that services in their branches were intermittent.
The latest disruption came just a day after Barclays customers were left struggling to log in.
While the firm claims its mobile app is working as normal, telling customers on Twitter that they "can still make payments and check your balance in our Mobile Banking app as normal", INQ readers tell us that the app throwing up error messages when they attempt to access transactions.
A spokeswoman for Barclays said they were working to fix the problems. The manager was trying to placate customers and couldn't advise when they would be back online.
The latest banking problems follow TSB's huge IT meltdown earlier this year, following a botched IT switch which saw customers locked out of their accounts.
A number of business customers have complained that they are now unable to organise tomorrow's payments for staff.
The service status report on the Barclays website confirms there is an outage of both online and telephone banking.